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cav 3 2  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology cav 3 2
    Cav 3 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 257 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cav+3+2/ppr0892348-195-16-27?v=Santa+Cruz+Biotechnology
    Average 94 stars, based on 257 article reviews
    cav 3 2 - by Bioz Stars, 2026-08
    94/100 stars

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    Image Search Results


    A) Caveolin protein levels were investigated using human cell lines and mouse primary cultures. Cells were seeded in 60-mm dishes (at a density of 12×10 4 ) and cultured in GM until confluence, followed by incubation in DM. After 72 hours in GM or DM, cells were harvested and protein homogenates western blotted for pCav-1, Cav-1, Cav-2, Cav-3 and tubulin. Protein bands were quantified by densitometry after normalization with respect to tubulin (n = 3). *, P<0.05; **, P<0.001; ***, P<0.0001. B) Human RD cells were seeded in 60-mm dishes (at a density of 15×10 4 ). After 24 hours, cells were treated or not with 10 ng/ml HGF for 24 hours, in the absence or presence of pre-administered 10 µM PP2. Cells were then harvested and protein homogenates western blotted for pSrc, pCav-1, Cav-1 and tubulin. Protein bands were quantified by densitometry after normalization with respect to tubulin (n = 3). *, P<0.05. After 48 hours, cell proliferation was measured by Crystal violet assay. Histograms represent means ± SD of absorbance. (n = 4) *, P<0.05.

    Journal: PLoS ONE

    Article Title: Phosphocaveolin-1 Enforces Tumor Growth and Chemoresistance in Rhabdomyosarcoma

    doi: 10.1371/journal.pone.0084618

    Figure Lengend Snippet: A) Caveolin protein levels were investigated using human cell lines and mouse primary cultures. Cells were seeded in 60-mm dishes (at a density of 12×10 4 ) and cultured in GM until confluence, followed by incubation in DM. After 72 hours in GM or DM, cells were harvested and protein homogenates western blotted for pCav-1, Cav-1, Cav-2, Cav-3 and tubulin. Protein bands were quantified by densitometry after normalization with respect to tubulin (n = 3). *, P<0.05; **, P<0.001; ***, P<0.0001. B) Human RD cells were seeded in 60-mm dishes (at a density of 15×10 4 ). After 24 hours, cells were treated or not with 10 ng/ml HGF for 24 hours, in the absence or presence of pre-administered 10 µM PP2. Cells were then harvested and protein homogenates western blotted for pSrc, pCav-1, Cav-1 and tubulin. Protein bands were quantified by densitometry after normalization with respect to tubulin (n = 3). *, P<0.05. After 48 hours, cell proliferation was measured by Crystal violet assay. Histograms represent means ± SD of absorbance. (n = 4) *, P<0.05.

    Article Snippet: Antibodies used for immunoblotting/immunohistochemistry were phospho-Cav-1 (Tyr14) (code 61338, BD Biosciences, Buccinasco, Italy), Cav-1 (code SC-894, Santa Cruz Biotechnology, Dallas, USA), Cav-2 and Cav-3 (code 610684 and 610420, BD Biosciences, Buccinasco, Italy), phosho-Src (Tyr418) (code 569732, Merck-Millipore, Milan, Italy), total and phospho-AKT (Ser473) (code #2920 and #4060, Cell Signalling, Milan, Italy), total and phospho-ERK (Tyr204) (code SC-135900 and SC-7883, Santa Cruz Biotechnology, Dallas, USA), MKI67 (code NCL-L-Ki67-MM1, NovocastraTM Laboratories Ltd, Newcastle Upon Tyne, United Kingdom), CD31 (code SC-8306, Santa Cruz Biotechnology, Dallas, USA), cleaved Caspase-3 (code #9661, Cell Signalling, Milan, Italy) and tubulin (code T5168, Sigma-Aldrich, Milan, Italy).

    Techniques: Cell Culture, Incubation, Western Blot, Crystal Violet Assay